Ammonium persulfate
About This Item
Linear Formula:
(NH4)2S2O8
CAS Number:
7727-54-0
Molecular Weight:
228.20
General description
Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.
Quality Segment: 200
Vapor Density: 7.9 (vs air)
Assay: ≥98%
Form: Powder
Reaction Suitability: reagent type: oxidant
Techniques: electrophoresis: suitable
Anion Traces: chloride (Cl-): <10 ppm
Cation Traces: Fe: <10 ppm, heavy metals (as Pb): <50 ppm
Foreign Activity: DNAse, none detected, Protease, none detected, RNAse, none detected
SMILES String: N.N.OS(=O)(=O)OOS(O)(=O)=O
